Title | Adhesion strength-based, label-free isolation of human pluripotent stem cells. |
Publication Type | Journal Article |
Year of Publication | 2013 |
Authors | Singh, A, Suri, S, Lee, T, Chilton, JM, Cooke, MT, Chen, W, Fu, J, Stice, SL, Lu, H, McDevitt, TC, García, AJ |
Journal | Nature Methods |
Date Published | May 2013 |
ISSN | 1548-7105 |
Keywords | Cell Adhesion, Cell Differentiation, Cell Separation, Humans, Karyotyping, Pluripotent Stem Cells |
Abstract | We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over ∼10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells. |
DOI | 10.1038/nmeth.2437 |
Alternate Journal | Nat. Methods |
PubMed ID | 23563795 |
PubMed Central ID | PMC3641175 |
Grant List | RC1 CA144825 / CA / NCI NIH HHS / United States R43 NS080407 / NS / NINDS NIH HHS / United States R43 NS080407 / NS / NINDS NIH HHS / United States R01 GM065918 / GM / NIGMS NIH HHS / United States RC1CA144825 / CA / NCI NIH HHS / United States |