TitleAdhesion strength-based, label-free isolation of human pluripotent stem cells.
Publication TypeJournal Article
Year of Publication2013
AuthorsSingh, A, Suri, S, Lee, T, Chilton, JM, Cooke, MT, Chen, W, Fu, J, Stice, SL, Lu, H, McDevitt, TC, García, AJ
JournalNature Methods
Date PublishedMay 2013
ISSN1548-7105
KeywordsCell Adhesion, Cell Differentiation, Cell Separation, Humans, Karyotyping, Pluripotent Stem Cells
Abstract

We demonstrate substantial differences in 'adhesive signature' between human pluripotent stem cells (hPSCs), partially reprogrammed cells, somatic cells and hPSC-derived differentiated progeny. We exploited these differential adhesion strengths to rapidly (over ∼10 min) and efficiently isolate fully reprogrammed induced hPSCs (hiPSCs) as intact colonies from heterogeneous reprogramming cultures and from differentiated progeny using microfluidics. hiPSCs were isolated label free, enriched to 95%-99% purity with >80% survival, and had normal transcriptional profiles, differentiation potential and karyotypes. We also applied this strategy to isolate hPSCs (hiPSCs and human embryonic stem cells) during routine culture and show that it may be extended to isolate hPSC-derived lineage-specific stem cells or differentiated cells.

DOI10.1038/nmeth.2437
Alternate JournalNat. Methods
PubMed ID23563795
PubMed Central IDPMC3641175
Grant ListRC1 CA144825 / CA / NCI NIH HHS / United States
R43 NS080407 / NS / NINDS NIH HHS / United States
R43 NS080407 / NS / NINDS NIH HHS / United States
R01 GM065918 / GM / NIGMS NIH HHS / United States
RC1CA144825 / CA / NCI NIH HHS / United States