TitleSmart and biofunctional streptavidin.
Publication TypeJournal Article
Year of Publication1999
AuthorsStayton, PS, Nelson, KE, McDevitt, TC, Bulmus, V, Shimoboji, T, Ding, Z, Hoffman, AS
JournalBiomolecular Engineering
Date PublishedDecember 1999
KeywordsAffinity Labels, Binding Sites, Biotechnology, Biotin, Models, Molecular, Protein Conformation, Protein Engineering, Protein Structure, Quaternary, Recombinant Fusion Proteins, Streptavidin

The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. While it is extremely useful as the native protein, there are many applications where its function can be improved re-engineering the subunits. We review here our efforts to construct streptavidin tetramers that have 'smart' recognition capabilities, and which display functional peptide sequences. These smart and biofunctional streptavidin derivatives can 'talk' to cells, and 'listen' to external signals which control capture and release of biotinylated molecules.

Alternate JournalBiomol. Eng.
PubMed ID10796990
Grant ListRR01296 / RR / NCRR NIH HHS / United States
DK49655 / DK / NIDDK NIH HHS / United States