|Histone h1 depletion impairs embryonic stem cell differentiation.
|Year of Publication
|Zhang, Y, Cooke, MT, Panjwani, S, Cao, K, Krauth, B, Ho, P-Y, Medrzycki, M, Berhe, DT, Pan, C, McDevitt, TC, Fan, Y
|Animals, Cell Differentiation, Cells, Cultured, Chromatin, DNA Methylation, Embryonic Development, Embryonic Stem Cells, Epigenesis, Genetic, Gene Expression Regulation, Developmental, Histones, Mice, Neurites, Neurons, Octamer Transcription Factor-3, Promoter Regions, Genetic
Pluripotent embryonic stem cells (ESCs) are known to possess a relatively open chromatin structure; yet, despite efforts to characterize the chromatin signatures of ESCs, the role of chromatin compaction in stem cell fate and function remains elusive. Linker histone H1 is important for higher-order chromatin folding and is essential for mammalian embryogenesis. To investigate the role of H1 and chromatin compaction in stem cell pluripotency and differentiation, we examine the differentiation of embryonic stem cells that are depleted of multiple H1 subtypes. H1c/H1d/H1e triple null ESCs are more resistant to spontaneous differentiation in adherent monolayer culture upon removal of leukemia inhibitory factor. Similarly, the majority of the triple-H1 null embryoid bodies (EBs) lack morphological structures representing the three germ layers and retain gene expression signatures characteristic of undifferentiated ESCs. Furthermore, upon neural differentiation of EBs, triple-H1 null cell cultures are deficient in neurite outgrowth and lack efficient activation of neural markers. Finally, we discover that triple-H1 null embryos and EBs fail to fully repress the expression of the pluripotency genes in comparison with wild-type controls and that H1 depletion impairs DNA methylation and changes of histone marks at promoter regions necessary for efficiently silencing pluripotency gene Oct4 during stem cell differentiation and embryogenesis. In summary, we demonstrate that H1 plays a critical role in pluripotent stem cell differentiation, and our results suggest that H1 and chromatin compaction may mediate pluripotent stem cell differentiation through epigenetic repression of the pluripotency genes.
|PubMed Central ID
|R01 GM085261 / GM / NIGMS NIH HHS / United States
GM085261 / GM / NIGMS NIH HHS / United States
CBET-0939511 / / PHS HHS / United States