|Innovation in the culture and derivation of pluripotent human stem cells.
|Year of Publication
|McDevitt, TC, Palecek, SP
|Current Opinion in Biotechnology
|Biotechnology, Cell Culture Techniques, Humans, Pluripotent Stem Cells, Tissue Engineering
In recent years, substantial progress has been made in identifying culture conditions and specific molecular factors that maintain human embryonic stem cells (hESCs) in a self-renewing, pluripotent state. As science and medicine move closer to producing viable hESC-based therapeutics, effective methods of isolating and maintaining undifferentiated hESCs using clinically acceptable good manufacturing practices must be developed. In recent years, progress toward this goal has included the identification of molecular factors that induce or repress hESC self-renewal and the development of defined media that support long-term hESC expansion. In addition, the recent discovery of novel means to derive pluripotent cells that avoid embryo destruction, including induced pluripotent stem (iPS cells), may mitigate ethical concerns associated with the use of hESCs.
|Curr. Opin. Biotechnol.
|PubMed Central ID
|R01 EB007534-02 / EB / NIBIB NIH HHS / United States
R01 EB007534 / EB / NIBIB NIH HHS / United States
1R01EB007534 / EB / NIBIB NIH HHS / United States
R21EB007316 / EB / NIBIB NIH HHS / United States