Title | Smart and biofunctional streptavidin. |
Publication Type | Journal Article |
Year of Publication | 1999 |
Authors | Stayton, PS, Nelson, KE, McDevitt, TC, Bulmus, V, Shimoboji, T, Ding, Z, Hoffman, AS |
Journal | Biomolecular Engineering |
Date Published | December 1999 |
ISSN | 1389-0344 |
Keywords | Affinity Labels, Binding Sites, Biotechnology, Biotin, Models, Molecular, Protein Conformation, Protein Engineering, Protein Structure, Quaternary, Recombinant Fusion Proteins, Streptavidin |
Abstract | The high affinity recognition of biotin and biotinylated molecules has made streptavidin one of the most important components in diagnostics and laboratory kits. While it is extremely useful as the native protein, there are many applications where its function can be improved re-engineering the subunits. We review here our efforts to construct streptavidin tetramers that have 'smart' recognition capabilities, and which display functional peptide sequences. These smart and biofunctional streptavidin derivatives can 'talk' to cells, and 'listen' to external signals which control capture and release of biotinylated molecules. |
Alternate Journal | Biomol. Eng. |
PubMed ID | 10796990 |
Grant List | RR01296 / RR / NCRR NIH HHS / United States DK49655 / DK / NIDDK NIH HHS / United States |