Based on the potential of stem cells to induce repair of injured and diseased tissues, we aim to harness the intrinsic therapeutic potential by delivering stem cells, stem cell-derived materials and secreted morphogenic factors to explore applications in immunomodulation and tissue regeneration.

Cell Delivery  - Stem cell 3D culture and differentiation system represents a novel platform of generating micro-tissues. We have established platforms for 3D expansion and differentiation of embryonic and mesenchymal stem cell aggregates culture system to generate uniform and high-throughput micro-tissues that are suitable for injectable delivery in vivo. Our efforts are leading towards repair of cartilage micro-defects and immunomodulation in animal models.

Stem Cell Derived Materials - Embryonic stem cells undergoing differentiation produce abundant extracellular matrix consisting of variety of growth factors and structurally proteins, which can be used to yield “embryonic-like”, scarless tissue regeneration in adult mammalian species. We have successfully developed stem cell-derived materials from differentiated embryonic stem cell aggregates using multiple decellularization techniques. Ultimately, stem cell-derived materials are expected to augment endogenous repair mechanisms in in animal defect models for tissue regeneration, such as bone formation and wound healing.

Secreted Factor Delivery - Morphogenic factors produced by stem cells are attractive therapeutic molecules for regenerative medicine. We are working in parallel to characterize and harness the temporal morphogen profile associated with stem cell differentiation at genomic, proteomic and glycomic levels to understand the key factors implicated in morphogenesis and differentiation and ultimately to develop engineering techniques for delivery of stem cell secreted morphogens in vitro and in vivo to control cell differentiation and tissue regeneration.

Related Publications

Associated Collaborators

College of Engineering, Virginia Commonwealth University
Surgery, Division of Vascular Surgery, Department of Surgery, Emory University School of Medicine
UCSF School of Medicine, Gladstone Institutes
Mechanical Engineering, Georgia Institute of Technology
Department of Neurological Surgery, UCSF
Biomedical Engineering, Georgia Tech
Hope Heart Matrix Biology Program, Benaroya Research Institute